Elution®eneration
When the chromatographic medium is exhausted, it should be eluted. The basic principle is to desorb the target product by a more active ion or group. Different sorbents are different in their activity. Therefore, a suitable eluent should be selected to elute the protein from chromatography media. There are roughly three ways to stepwise elution ion exchange chromatography : One is for simultaneous elution. The eluent is a dilute acid, alkali or salt solution may be used, and an appropriate organic solvent may also be used, wherein the salt solution is mainly used, and the dosage form according to the nature of the target product and the final product is selected. Since the substance to be absorbed is often not a single species, the charge of each substance is different, and the binding strength to the medium is different. Even if the same eluent is used, the easily replaced substance will flow out of the medium first, and the binding force is strong. After the material is discharged